Publisher Summary This chapter discusses preparation of isolated rat liver cells. The early
mechanical and chemical methods for liver-cell preparation were relatively successful in
converting liver tissue to a suspension of isolated cells. The successful preparation of intact
liver cells by perfusion with collagenase is technically quite difficult. The major method for
preparation of nonparenchymal liver cells is based on the selective sensitivity of
parenchymal cells toward proteases. By incubation of rat liver minces with pronase, most of …