[HTML][HTML] Molecular and cellular physiology of apolipoprotein AI lipidation by the ATP-binding cassette transporter A1 (ABCA1)
The dynamics of ABCA1-mediated apoA-I lipidation were investigated in intact human
fibroblasts induced with 22 (R)-hydroxycholesterol and 9-cis-retinoic acid (stimulated cells).
Specific binding parameters of 125 I-apoA-I to ABCA1 at 37° C were determined: K d= 0.65
μg/ml, B max= 0.10 ng/μg cell protein. Lipid-free apoA-I inhibited the binding of 125 I-apoA-I
to ABCA1 more efficiently than pre-β 1-LpA-I, reconstituted HDL particles r (LpA-I), or HDL 3
(IC 50= 0.35±1.14, apoA-I; 1.69±1.07, pre-β 1-LpA-I; 17.91±1.39, r (LpA-I); and 48.15±1.72 …
fibroblasts induced with 22 (R)-hydroxycholesterol and 9-cis-retinoic acid (stimulated cells).
Specific binding parameters of 125 I-apoA-I to ABCA1 at 37° C were determined: K d= 0.65
μg/ml, B max= 0.10 ng/μg cell protein. Lipid-free apoA-I inhibited the binding of 125 I-apoA-I
to ABCA1 more efficiently than pre-β 1-LpA-I, reconstituted HDL particles r (LpA-I), or HDL 3
(IC 50= 0.35±1.14, apoA-I; 1.69±1.07, pre-β 1-LpA-I; 17.91±1.39, r (LpA-I); and 48.15±1.72 …