Discovery and analysis of antisense oligonucleotide activity in cell culture
BF Baker, TP Condon, E Koller, RA McKay… - Methods, 2001 - Elsevier
BF Baker, TP Condon, E Koller, RA McKay, AM Siwkowski, TA Vickers, BP Monia
Methods, 2001•ElsevierIn the past decade antisense oligonucleotides (ASOs) have proven to be a useful tool for
dissection of gene function in molecular cell biology (Koller, E., Gaarde, WA, and Monia, BP
(2000) Trends Pharm. Sci., 21, 142–148), and validation of gene targets in animal models
(Crooke, ST (1998) Biotechnol. Gen. Eng. Rev. 15, 121–157), as well as a means for
therapeutic treatment of human diseases (Bennett, CF (1999) Exp. Opin. Invest. Drugs8, 237–
253). An important step toward usage of ASOs in the described applications is identification …
dissection of gene function in molecular cell biology (Koller, E., Gaarde, WA, and Monia, BP
(2000) Trends Pharm. Sci., 21, 142–148), and validation of gene targets in animal models
(Crooke, ST (1998) Biotechnol. Gen. Eng. Rev. 15, 121–157), as well as a means for
therapeutic treatment of human diseases (Bennett, CF (1999) Exp. Opin. Invest. Drugs8, 237–
253). An important step toward usage of ASOs in the described applications is identification …
In the past decade antisense oligonucleotides (ASOs) have proven to be a useful tool for dissection of gene function in molecular cell biology (Koller, E., Gaarde, W. A., and Monia, B. P. (2000) Trends Pharm. Sci.,21, 142–148), and validation of gene targets in animal models (Crooke, S. T. (1998) Biotechnol. Gen. Eng. Rev.15, 121–157), as well as a means for therapeutic treatment of human diseases (Bennett, C. F. (1999) Exp. Opin. Invest. Drugs8, 237–253). An important step toward usage of ASOs in the described applications is identification of an active ASO. This article describes the underlying basis and means for achieving this goal in cell culture.
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