[HTML][HTML] Dynamics of insulin signaling in 3T3-L1 adipocytes: differential compartmentalization and trafficking of insulin receptor substrate (IRS)-1 and IRS-2
G Inoue, B Cheatham, R Emkey, CR Kahn - Journal of Biological Chemistry, 1998 - ASBMB
The ability of the insulin receptor to phosphorylate multiple substrates and their subcellular
localization are two of the determinants that contribute to diversity of signaling. We find that
insulin receptor substrate (IRS)-1 is 2-fold more concentrated in the intracellular membrane
(IM) compartment than in cytosol, whereas IRS-2 is 2-fold more concentrated in cytosol than
in IM. Insulin stimulation induces rapid tyrosine phosphorylation of both IRS-1 and IRS-2.
This occurs mainly in the IM compartment, even though IRS-2 is located predominantly in …
localization are two of the determinants that contribute to diversity of signaling. We find that
insulin receptor substrate (IRS)-1 is 2-fold more concentrated in the intracellular membrane
(IM) compartment than in cytosol, whereas IRS-2 is 2-fold more concentrated in cytosol than
in IM. Insulin stimulation induces rapid tyrosine phosphorylation of both IRS-1 and IRS-2.
This occurs mainly in the IM compartment, even though IRS-2 is located predominantly in …