[PDF][PDF] Long-term hematopoietic stem cells require stromal cell-derived factor-1 for colonizing bone marrow during ontogeny

T Ara, K Tokoyoda, T Sugiyama, T Egawa, K Kawabata… - Immunity, 2003 - cell.com
T Ara, K Tokoyoda, T Sugiyama, T Egawa, K Kawabata, T Nagasawa
Immunity, 2003cell.com
The physiological role of SDF-1 on hematopoietic stem cells (HSCs) remains elusive. We
show that colonization of bone marrow by HSCs in addition to myeloid cells is severely
impaired in SDF-1−/− embryos by a long-term repopulation assay. Colonization of spleen by
HSCs was also affected, but to a lesser extent. Enforced expression of SDF-1 under the
control of vascular-specific Tie-2 regulatory sequences could completely rescue the
reduction of HSCs but not myeloid cells in SDF-1−/− bone marrow. SDF-1 was detected in …
Abstract
The physiological role of SDF-1 on hematopoietic stem cells (HSCs) remains elusive. We show that colonization of bone marrow by HSCs in addition to myeloid cells is severely impaired in SDF-1−/− embryos by a long-term repopulation assay. Colonization of spleen by HSCs was also affected, but to a lesser extent. Enforced expression of SDF-1 under the control of vascular-specific Tie-2 regulatory sequences could completely rescue the reduction of HSCs but not myeloid cells in SDF-1−/− bone marrow. SDF-1 was detected in the vicinity of the vascular endothelial cells in fetal bone marrow. SDF-1 plays a critical role in colonization of bone marrow by HSCs and myeloid cells during ontogeny, and the mechanisms by which SDF-1 functions are distinct between HSCs and myeloid cells.
cell.com