Three-dimensional localization of ultrasmall immuno-gold labels by HAADF-STEM tomography

U Ziese, C Kübel, AJ Verkleij, AJ Koster - Journal of structural biology, 2002 - Elsevier
The localization of scarce antigens in thin sections of biological material can be
accomplished by pre-embedment labeling with ultrasmall immuno-gold labels. Moreover,
with this method, labeling is not restricted to the section surface but occurs throughout the
section volume. Thus, when combined with electron tomography, antigens can be localized
in three dimensions in relation to the 3D (three-dimensional) ultrastructure of the cell.
However, for visualization in a transmission electron microscope, these labels need to be …