Functional analysis of human T cell subsets defined by monoclonal antibodies. IV. Induction of suppressor cells within the OKT4+ population.
Y Thomas, L Rogozinski, OH Irigoyen… - Journal of Experimental …, 1981 - rupress.org
Y Thomas, L Rogozinski, OH Irigoyen, SM Friedman, PC Kung, G Goldstein, L Chess
Journal of Experimental Medicine, 1981•rupress.orgMaterials and Methods Lymphocyte Preparation andIsolation ofHuman TandB Cells. Fresh
peripheral blood lymphocytes were isolated from consenting healthy human volunteers by
Ficoll-Hypaque density gradient centrifugation. Highly enriched population of T and B cells
were then isolated by methods previously described in detail (10). In brief, human
mononuclear cells were washed in minimum essential medium (Grand Island Biological Co.,
Grand Island, NY) containing 5% fetal calf serum (FCS; Microbiological Associates …
peripheral blood lymphocytes were isolated from consenting healthy human volunteers by
Ficoll-Hypaque density gradient centrifugation. Highly enriched population of T and B cells
were then isolated by methods previously described in detail (10). In brief, human
mononuclear cells were washed in minimum essential medium (Grand Island Biological Co.,
Grand Island, NY) containing 5% fetal calf serum (FCS; Microbiological Associates …
Materials and Methods
Lymphocyte Preparation andIsolation ofHuman TandB Cells. Fresh peripheral blood lymphocytes were isolated from consenting healthy human volunteers by Ficoll-Hypaque density gradient centrifugation. Highly enriched population of T and B cells were then isolated by methods previously described in detail (10). In brief, human mononuclear cells were washed in minimum essential medium (Grand Island Biological Co., Grand Island, NY) containing 5% fetal calf serum (FCS; Microbiological Associates, Bethesda, Md.) and then separated into surface Ig
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