RNA interference in adult mice
AP McCaffrey, L Meuse, TTT Pham, DS Conklin… - Nature, 2002 - nature.com
Nature, 2002•nature.com
RNA interference is an evolutionarily conserved surveillance mechanism that responds to
double-stranded RNA by sequence-specific silencing of homologous genes. Here we show
that transgene expression can be suppressed in adult mice by synthetic small interfering
RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the
therapeutic potential of this technique by demonstrating effective targeting of a sequence
from hepatitis C virus by RNA interference in vivo.
double-stranded RNA by sequence-specific silencing of homologous genes. Here we show
that transgene expression can be suppressed in adult mice by synthetic small interfering
RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the
therapeutic potential of this technique by demonstrating effective targeting of a sequence
from hepatitis C virus by RNA interference in vivo.
Abstract
RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.
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