The human endothelial cell in tissue culture

Y Maruyama - Zeitschrift für Zellforschung und Mikroskopische …, 1963 - Springer
Y Maruyama
Zeitschrift für Zellforschung und Mikroskopische Anatomie, 1963Springer
Endothelial cells of the human umbilical vein were isolated by trypsin and cultured.
Histological preparations of those umbilical cords were made to check the removal of the
endothelium and not of other tissues. The cells in culture grew in sheets which were
identified with endothelial cells. Isolated cells rarely showed a spindle shape. Further
investigation is needed to distinguish spindle-shaped cells from fibroblasts or other cellular
elements. Identification of endothelial cells in the previous reports and materials used are …
Summary
Endothelial cells of the human umbilical vein were isolated by trypsin and cultured. Histological preparations of those umbilical cords were made to check the removal of the endothelium and not of other tissues.
The cells in culture grew in sheets which were identified with endothelial cells. Isolated cells rarely showed a spindle shape. Further investigation is needed to distinguish spindle-shaped cells from fibroblasts or other cellular elements.
Identification of endothelial cells in the previous reports and materials used are discussed.
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