Simplified northern blot hybridization using 5% sodium dodecyl sulfate.

GD Virca, W Northemann, BR Shiels, G Widera… - …, 1990 - europepmc.org
GD Virca, W Northemann, BR Shiels, G Widera, S Broome
Biotechniques, 1990europepmc.org
We describe a simplified Northern blot hybridization procedure. This procedure eliminates
the need for many reagents commonly used in RNA hybridization and replaces them with
two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all
post-hybridization washes.
We describe a simplified Northern blot hybridization procedure. This procedure eliminates the need for many reagents commonly used in RNA hybridization and replaces them with two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all post-hybridization washes.
europepmc.org