Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes.
SM Fu, N Chiorazzi, HG Kunkel, JP Halper… - Journal of Experimental …, 1978 - rupress.org
SM Fu, N Chiorazzi, HG Kunkel, JP Halper, SR Harris
Journal of Experimental Medicine, 1978•rupress.orgMaterials and Methods Isolation ofLymphocytes. Mononuclear cells were isolated by Ficoll-
Hypaque gradients (Pharmacia Fine Chemicals, Piscataway, NJ) from the peripheral blood
of normal individuals and patients with CLL. Tonsillar mononuclear cells were isolated as
described previously (7). Spontaneous rosette formation between human lymphocytes and
sheep erythrocytes (SRBC) was performed with neuraminidase-treated SRBC (EN). The E
rosette-forming cells were separated from the nonrosette" forming cells by Ficoll-Hypaque …
Hypaque gradients (Pharmacia Fine Chemicals, Piscataway, NJ) from the peripheral blood
of normal individuals and patients with CLL. Tonsillar mononuclear cells were isolated as
described previously (7). Spontaneous rosette formation between human lymphocytes and
sheep erythrocytes (SRBC) was performed with neuraminidase-treated SRBC (EN). The E
rosette-forming cells were separated from the nonrosette" forming cells by Ficoll-Hypaque …
Materials and Methods
Isolation ofLymphocytes. Mononuclear cells were isolated by Ficoll-Hypaque gradients (Pharmacia Fine Chemicals, Piscataway, NJ) from the peripheral blood of normal individuals and patients with CLL. Tonsillar mononuclear cells were isolated as described previously (7). Spontaneous rosette formation between human lymphocytes and sheep erythrocytes (SRBC) was performed with neuraminidase-treated SRBC (EN). The E rosette-forming cells were separated from the nonrosette" forming cells by Ficoll-Hypaque gradient centrifugation. The rosette-forming cell fraction was purified further by a second density gradient centrifugation. Repeated gradient centrifugation was performed until no significant cells were observed at the
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