Collagen biosynthesis by human skin fibroblasts. I. Optimization of the culture conditions for synthesis of type I and type III procollagens
BA Booth, KL Polak, J Uitto - Biochimica et Biophysica Acta (BBA)-Nucleic …, 1980 - Elsevier
Skin fibroblasts in culture can provide a convenient means to study aberrations of collagen
metabolism in a variety of clinical conditions. In the present study, the culture conditions for
the synthesis of procollagen by cultured human skin fibroblasts were optimized by
independently varying parameters in the cell culture environment. To study the synthesis of
procollagen the cell cultures were labeled with [3 H] proline and the collagenous
polypeptides were determined either by measuring the synthesis of hydroxy [3 H] proline or …
metabolism in a variety of clinical conditions. In the present study, the culture conditions for
the synthesis of procollagen by cultured human skin fibroblasts were optimized by
independently varying parameters in the cell culture environment. To study the synthesis of
procollagen the cell cultures were labeled with [3 H] proline and the collagenous
polypeptides were determined either by measuring the synthesis of hydroxy [3 H] proline or …
