Regulation of intracellular polymorphonuclear leukocyte Fc receptors by lipopolysaccharide
HH Simms, R D'Amico - Cellular immunology, 1994 - Elsevier
Endotoxemia, in man, has been associated with an autooxidative reduction in the
bioavailability of polymorphonuclear leukocyte receptors. The location and mechanisms of
this phenomena have remained unclear; we investigated the effects of lipopolysaccharide
(LPS) on intracellular Fcγ receptor expression. Polymorphonuclear leukocytes (PMN) were
incubated with LPS (10 ng/ml), permeabilized with saponin, followed by measurement of
CD64, CD32w, and CD16 (FcγRI, II, III) using 125 I-monoclonal antibodies directed against …
bioavailability of polymorphonuclear leukocyte receptors. The location and mechanisms of
this phenomena have remained unclear; we investigated the effects of lipopolysaccharide
(LPS) on intracellular Fcγ receptor expression. Polymorphonuclear leukocytes (PMN) were
incubated with LPS (10 ng/ml), permeabilized with saponin, followed by measurement of
CD64, CD32w, and CD16 (FcγRI, II, III) using 125 I-monoclonal antibodies directed against …