Two rapid and simple methods used for the removal of resins from 1.0 μm thick epoxy sections
MH Maxwell - Journal of Microscopy, 1978 - Wiley Online Library
MH Maxwell
Journal of Microscopy, 1978•Wiley Online LibraryUltrathin epoxy sections are commonly used in electron microscopy. More useful information
can often be obtained by examining thicker (0.5–3.0 μm) resin sections under the light
microscope. However, there are some histological stains that are unable to penetrate these
resins. This short note describes the easy and rapid preparation of two resin‐removal
solutions which allow subsequent staining of 1.0 μm thick sections without obvious tissue
damage and with excellent results using standard histological dyes.
can often be obtained by examining thicker (0.5–3.0 μm) resin sections under the light
microscope. However, there are some histological stains that are unable to penetrate these
resins. This short note describes the easy and rapid preparation of two resin‐removal
solutions which allow subsequent staining of 1.0 μm thick sections without obvious tissue
damage and with excellent results using standard histological dyes.
Summary
Ultrathin epoxy sections are commonly used in electron microscopy. More useful information can often be obtained by examining thicker (0.5–3.0 μm) resin sections under the light microscope. However, there are some histological stains that are unable to penetrate these resins.
This short note describes the easy and rapid preparation of two resin‐removal solutions which allow subsequent staining of 1.0 μm thick sections without obvious tissue damage and with excellent results using standard histological dyes.
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