Direct Colorimetric Determination of Serum Calcium with o-Cresolphthalein Complexon
LG Morin - American journal of clinical pathology, 1974 - academic.oup.com
LG Morin
American journal of clinical pathology, 1974•academic.oup.comA simple, rapid colorimetric procedure for measuring calcium with a single, stable o-
cresolphthalein complexon reagent is described. The procedure is accurate (r2= 0.96;
recovery= 98.8%), precise (CV= 1.9%) and sensitive (absorptivity= 740). The color
developed is stable for several hours. The procedure does not require deproteinization, and
phosphorus, magnesium, bilirubin, hemoglobin, and albumin do not interfere. Linearity
extends to 18 mg. per dl.
cresolphthalein complexon reagent is described. The procedure is accurate (r2= 0.96;
recovery= 98.8%), precise (CV= 1.9%) and sensitive (absorptivity= 740). The color
developed is stable for several hours. The procedure does not require deproteinization, and
phosphorus, magnesium, bilirubin, hemoglobin, and albumin do not interfere. Linearity
extends to 18 mg. per dl.
Abstract
A simple, rapid colorimetric procedure for measuring calcium with a single, stable o-cresolphthalein complexon reagent is described. The procedure is accurate (r2 = 0.96; recovery = 98.8%), precise (C.V. = 1.9%) and sensitive (absorptivity = 740). The color developed is stable for several hours. The procedure does not require deproteinization, and phosphorus, magnesium, bilirubin, hemoglobin, and albumin do not interfere. Linearity extends to 18 mg. per dl.
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