Cleavage of type VIII collagen by human neutrophil elastase
R Kittelberger, TJ Neale, KT Francky… - … et Biophysica Acta (BBA …, 1992 - Elsevier
R Kittelberger, TJ Neale, KT Francky, NS Greenhill, GJ Gibson
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1992•ElsevierIn this report, the susceptibility of type VIII collagen to human neutrophil elastase is
compared to other extracellular matrix components. Type X collagen is degraded specific
fragments at a substrate to enzyme ratio of 5: 1 after 20 h at room temperature, but type VIII
collagen is almost completely degraded after only 4 h incubation at a substrate to enzyme
ratio of 50: 1 and partly degraded after only 15 min. Laminin, merosin and types I, III, IV and
V collagen exhibit no susceptibility to neutrophil elastase under the latter conditions, while …
compared to other extracellular matrix components. Type X collagen is degraded specific
fragments at a substrate to enzyme ratio of 5: 1 after 20 h at room temperature, but type VIII
collagen is almost completely degraded after only 4 h incubation at a substrate to enzyme
ratio of 50: 1 and partly degraded after only 15 min. Laminin, merosin and types I, III, IV and
V collagen exhibit no susceptibility to neutrophil elastase under the latter conditions, while …
Abstract
In this report, the susceptibility of type VIII collagen to human neutrophil elastase is compared to other extracellular matrix components. Type X collagen is degraded specific fragments at a substrate to enzyme ratio of 5:1 after 20 h at room temperature, but type VIII collagen is almost completely degraded after only 4 h incubation at a substrate to enzyme ratio of 50:1 and partly degraded after only 15 min. Laminin, merosin and types I, III, IV and V collagen exhibit no susceptibility to neutrophil elastase under the latter conditions, while fibronectin is degraded.
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