Identification of α-galactosyl and other carbohydrate epitopes that are bound by human anti-pig antibodies: relevance to discordant xenografting in man

DKC Cooper, AH Good, E Koren, R Oriol… - Transplant …, 1993 - Elsevier
Human anti-pig antibodies were obtained by perfusing pig hearts (n= 4) and kidneys (n= 8)
with human AB or O plasma followed by elution with 3 M NaSCN. The antibodies were
screened against a panel of 132 synthetic carbohydrates conjugated to bovine serum
albumin using an enzyme-linked immunoassay. An anti-immunoglobulin antibody was also
used to detect immunoglobulin deposits on pig tissues. Four carbohydrate molecules with a
terminal α-galactose residue bound all but one of the human anti-pig kidney antibodies and …