[HTML][HTML] Contraction-induced changes in acetyl-CoA carboxylase and 5′-AMP-activated kinase in skeletal muscle
D Vavvas, A Apazidis, AK Saha, J Gamble… - Journal of Biological …, 1997 - ASBMB
The concentration of malonyl-CoA, a negative regulator of fatty acid oxidation, diminishes
acutely in contracting skeletal muscle. To determine how this occurs, the activity and
properties of acetyl-CoA carboxylase β (ACC-β), the skeletal muscle isozyme that catalyzes
malonyl-CoA formation, were examined in rat gastrocnemius-soleus muscles at rest and
during contractions induced by electrical stimulation of the sciatic nerve. To avoid the
problem of contamination of the muscle extract by mitochondrial carboxylases, an assay was …
acutely in contracting skeletal muscle. To determine how this occurs, the activity and
properties of acetyl-CoA carboxylase β (ACC-β), the skeletal muscle isozyme that catalyzes
malonyl-CoA formation, were examined in rat gastrocnemius-soleus muscles at rest and
during contractions induced by electrical stimulation of the sciatic nerve. To avoid the
problem of contamination of the muscle extract by mitochondrial carboxylases, an assay was …