[HTML][HTML] Measurement of parameters of cholic acid kinetics in plasma using a microscale stable isotope dilution technique: application to rodents and humans
CV Hulzebos, L Renfurm, RH Bandsma… - Journal of lipid …, 2001 - ASBMB
A stable isotope dilution method is described that allows measurement of cholic acid (CA)
kinetics, that is, pool size, fractional turnover rate (FTR), and synthesis rate in mice, rats, and
humans. Decay of administered [2, 2, 4, 4-2 H 4] CA enrichment was measured in time in 50-
μl plasma samples by gas-liquid chromatography/electron capture negative chemical
ionization-mass spectrometry, applying the pentafluorobenzyl-trimethylsilyl derivative. The
kinetic data expressed species-dependent differences. The CA pool sizes were 16.8±2.1 …
kinetics, that is, pool size, fractional turnover rate (FTR), and synthesis rate in mice, rats, and
humans. Decay of administered [2, 2, 4, 4-2 H 4] CA enrichment was measured in time in 50-
μl plasma samples by gas-liquid chromatography/electron capture negative chemical
ionization-mass spectrometry, applying the pentafluorobenzyl-trimethylsilyl derivative. The
kinetic data expressed species-dependent differences. The CA pool sizes were 16.8±2.1 …