Phagocytosis of opsonized oil droplets by neutrophils Adaptation to a microtiter plate format
H Rosen, BR Michel, A Chait - Journal of immunological methods, 1991 - Elsevier
A previously described assay for neutrophil phagocytosis of oil droplets labeled with the
lipophilic red dye, oil red O, has been adapted to a microtiter plate format. Oil laden
neutrophils are fixed to the plate with glutaraldehyde, washed free of uningested oil, and
evaluated for degree of oil red O uptake by direct determination of light absorption at 540 nm
in a microtiter plate reader. Advantages of the modified procedure include the ability to
determine rates of phagocytosis (multiple determinations) under diverse conditions …
lipophilic red dye, oil red O, has been adapted to a microtiter plate format. Oil laden
neutrophils are fixed to the plate with glutaraldehyde, washed free of uningested oil, and
evaluated for degree of oil red O uptake by direct determination of light absorption at 540 nm
in a microtiter plate reader. Advantages of the modified procedure include the ability to
determine rates of phagocytosis (multiple determinations) under diverse conditions …