Basal corticosterone levels of young ob/ob mice

PU Dubuc - Hormone and Metabolic Research, 1977 - thieme-connect.com
PU Dubuc
Hormone and Metabolic Research, 1977thieme-connect.com
Materials and Methods The experimental animals were obese and lean offspring of
heterozygote breeding pairs (C57BL/6J ob/+) purchased from Jackson Laboratories (Bar
Harbor, ME). The breeding pairs and offspring were housed in a colony room maintained at
23±20 and a 12 hour light cycle. Each litter was weaned at 21 days of age and sex-matched
littermates were housed (paired) in commercially available cages thereafter. At each age
sampled (as described in Results) 2 or 3 litters of 6 to 9 pups/litter were Iightly anaesthetized …
Materials and Methods
The experimental animals were obese and lean offspring of heterozygote breeding pairs (C57BL/6J ob/+) purchased from Jackson Laboratories (Bar Harbor, ME). The breeding pairs and offspring were housed in a colony room maintained at 23±20 and a 12 hour light cycle. Each litter was weaned at 21 days of age and sex-matched littermates were housed (paired) in commercially available cages thereafter. At each age sampled (as described in Results) 2 or 3 litters of 6 to 9 pups/litter were Iightly anaesthetized with ether and bled from the retroorbital plexus for the determination of glucose, IRI, and corticosterone. In the experiment each animal was bled only once, after wh ich it was marked and returned to its horne cage for phenotype determination at 5 weeks of age. Blood sampies were taken between 011.00 and 10.00 hours after one hour of food deprivation. The proeedures for the determination of glucose, IRI, and corticosterone were identical with those described earlier (Dubuc et al. 1975).
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