Expression and Immunogenicity of a Mutant Diphtheria Toxin Molecule, CRM197, and Its Fragments inSalmonella typhi Vaccine Strain CVD 908-htrA
N Orr, JE Galen, MM Levine - Infection and immunity, 1999 - Am Soc Microbiol
N Orr, JE Galen, MM Levine
Infection and immunity, 1999•Am Soc MicrobiolMutant diphtheria toxin molecule CRM197 and fragments thereof were expressed in
attenuated Salmonella typhi CVD 908-htrA, and the constructs were tested for their ability to
induce serum antitoxin. Initially, expressed proteins were insoluble, and the constructs failed
to induce neutralizing antitoxin. Soluble CRM197 was expressed at low levels by utilizing
the hemolysin A secretion system from Escherichia coli.
attenuated Salmonella typhi CVD 908-htrA, and the constructs were tested for their ability to
induce serum antitoxin. Initially, expressed proteins were insoluble, and the constructs failed
to induce neutralizing antitoxin. Soluble CRM197 was expressed at low levels by utilizing
the hemolysin A secretion system from Escherichia coli.
Abstract
Mutant diphtheria toxin molecule CRM197 and fragments thereof were expressed in attenuated Salmonella typhi CVD 908-htrA, and the constructs were tested for their ability to induce serum antitoxin. Initially, expressed proteins were insoluble, and the constructs failed to induce neutralizing antitoxin. Soluble CRM197 was expressed at low levels by utilizing the hemolysin A secretion system from Escherichia coli.
American Society for Microbiology