It has been previously reported that different quantitative results can be obtained when TNFα
is measured in biological fluids by bioassay and immunoassay. This is thought to be related
to the presence of antigenic forms of TNFα that cannot be detected by bioassay, such as
complexes with soluble receptors (sTNF-R) or TNFα monomers. In this work we have
observed discrepancies between antigenic and bioactive TNFα even when we used a
sandwich-ELISA, unable to detect TNFα monomers, based on antibodies that bind epitopes …