Therapy of disseminated murine leukemia with cyclophosphamide and immune Lyt-1+, 2-T cells. Tumor eradication does not require participation of cytotoxic T cells.
PD Greenberg, DE Kern, MA Cheever - Journal of Experimental …, 1985 - rupress.org
PD Greenberg, DE Kern, MA Cheever
Journal of Experimental Medicine, 1985•rupress.orgMaterials and Methods Mice. C57BL/6 (H-2 b, Thy-l. 2; denoted B6) mice, breeding pairs of
congenic B6. PL (74NS)(H-2 b, Thy-l. 1; denoted B6/Thy-l. 1), and BALB/c (H-2 d) mice were
obtained from The Jackson Laboratory, Bar Harbor, ME. To prepare ATXBM hosts, 5-wk-old
B6 mice were thymectomized, lethally irradiated (950 rad) 3 wk later, and injected
intravenously with B6 bone marrow cells that had been depleted of T cells by treatment with
monoclonal IgM anti-Thy-l. 2 plus selected rabbit complement. These mice, used 4 wk later …
congenic B6. PL (74NS)(H-2 b, Thy-l. 1; denoted B6/Thy-l. 1), and BALB/c (H-2 d) mice were
obtained from The Jackson Laboratory, Bar Harbor, ME. To prepare ATXBM hosts, 5-wk-old
B6 mice were thymectomized, lethally irradiated (950 rad) 3 wk later, and injected
intravenously with B6 bone marrow cells that had been depleted of T cells by treatment with
monoclonal IgM anti-Thy-l. 2 plus selected rabbit complement. These mice, used 4 wk later …
Materials and Methods Mice. C57BL/6 (H-2 b, Thy-l. 2; denoted B6) mice, breeding pairs of congenic B6. PL (74NS)(H-2 b, Thy-l. 1; denoted B6/Thy-l. 1), and BALB/c (H-2 d) mice were obtained from The Jackson Laboratory, Bar Harbor, ME. To prepare ATXBM hosts, 5-wk-old B6 mice were thymectomized, lethally irradiated (950 rad) 3 wk later, and injected intravenously with B6 bone marrow cells that had been depleted of T cells by treatment with monoclonal IgM anti-Thy-l. 2 plus selected rabbit complement. These mice, used 4 wk later, were killed at the end of the experiments to confirm the absence of Thy-1.2+ T cells.
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