[HTML][HTML] Cloning and functional expression of a thyrotropin receptor cDNA from rat fat cells
T Endo, K Ohta, K Haraguchi, T Onaya - Journal of Biological Chemistry, 1995 - ASBMB
Thyrotropin receptor (TSH-R) has been thought to be thyroid-specific, but, by Northern blot
analysis, we found that rat adipose tissue expressed TSH-R mRNAs in amounts
approaching those in the thyroid. To investigate the function of TSH-R from adipose tissue,
we screened a rat fat cell λgt11 cDNA library for TSH-R sequences using a 32 P-labeled rat
thyroid TSH-R cDNA as a probe. Among 10 6 plaques, we obtained four positive clones.
Sequencing of these cDNAs has revealed that two of them (Fα and Fβ) contained both …
analysis, we found that rat adipose tissue expressed TSH-R mRNAs in amounts
approaching those in the thyroid. To investigate the function of TSH-R from adipose tissue,
we screened a rat fat cell λgt11 cDNA library for TSH-R sequences using a 32 P-labeled rat
thyroid TSH-R cDNA as a probe. Among 10 6 plaques, we obtained four positive clones.
Sequencing of these cDNAs has revealed that two of them (Fα and Fβ) contained both …