[CITATION][C] A versatile method for the determination of serum cortisol binding globulin and sex hormone binding globulin binding capacities

GL Hammond, PLA Lähteenmäki - Clinica chimica acta, 1983 - Elsevier
GL Hammond, PLA Lähteenmäki
Clinica chimica acta, 1983Elsevier
Cortisol binding globulin (CBG) and sex hormone binding globulin (SHBG) are plasma
glycoproteins which bind some of the most important steroid hormones in blood with high
affinity and specificity, but limited capacity. Although antisera have been raised against
purified preparations of these proteins and radioimmunoassays have been developed to
measure their actual serum concentrations[1, 2], determinations of their steroid binding
capacities will continue to be of importance, especially if variants with abnormal steroid …
Cortisol binding globulin (CBG) and sex hormone binding globulin (SHBG) are plasma glycoproteins which bind some of the most important steroid hormones in blood with high affinity and specificity, but limited capacity. Although antisera have been raised against purified preparations of these proteins and radioimmunoassays have been developed to measure their actual serum concentrations[1, 2], determinations of their steroid binding capacities will continue to be of importance, especially if variants with abnormal steroid binding activities exist [3, 4]. In general, most methods currently used to determine the serum or plasma binding capacities of CBG and SHBG yield values which are in close agreement [5, 6]. However, some of these methods are not suited for the routine analysis of large numbers of samples [7, 8] while others require the use of expensive gels [9, 10], or work under cold room conditions[111. Ammonium sulphate precipitation is probably the most widely used method to determine serum SHBG binding capacity [12-141, but this technique is not so easily applied for measurement of CBG binding capacity, and cannot be readily adapted to measure SHBG binding capacity in samples containing low concentrations of protein, such as obtained during purification procedures. It has been our intention therefore to develop a reliable, inexpensive, versatile method that is suitable for the routine determination of both CBG and SHBG in dilute serum samples.
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