[HTML][HTML] Affinity, kinetics, and thermodynamics of E-selectin binding to E-selectin ligand-1
MK Wild, MC Huang, U Schulze-Horsel… - Journal of Biological …, 2001 - ASBMB
E-selectin is an endothelial adhesion molecule, which mediates the tethering and rolling of
leukocytes on vascular endothelium. It recognizes the glycoprotein E-selectin ligand-1 (ESL-
1) as a major binding partner on mouse myeloid cells. Using surface plasmon resonance,
we measured the kinetics and affinity of binding of monomeric E-selectin to ESL-1 isolated
from mouse bone marrow cells. E-selectin bound to ESL-1 with a fast dissociation rate
constant of 4.6 s− 1 and a calculated association rate constant of 7.4× 10 4 m− 1 s− 1. We …
leukocytes on vascular endothelium. It recognizes the glycoprotein E-selectin ligand-1 (ESL-
1) as a major binding partner on mouse myeloid cells. Using surface plasmon resonance,
we measured the kinetics and affinity of binding of monomeric E-selectin to ESL-1 isolated
from mouse bone marrow cells. E-selectin bound to ESL-1 with a fast dissociation rate
constant of 4.6 s− 1 and a calculated association rate constant of 7.4× 10 4 m− 1 s− 1. We …