[HTML][HTML] Post-translational Modifications of Recombinant P-selectin Glycoprotein Ligand-1 Required for Binding to P-and E-selectin (∗)
F Li, PP Wilkins, S Crawley, J Weinstein… - Journal of Biological …, 1996 - ASBMB
P-selectin glycoprotein ligand-1 (PSGL-1) is a mucin-like ligand for P-and E-selectin on
human leukocytes. PSGL-1 requires sialylated, fucosylated O-linked glycans and tyrosine
sulfate to bind P-selectin. Less is known about the determinants that PSGL-1 requires to
bind E-selectin. To further define the modifications required for PSGL-1 to bind P-and E-
selectin, we transfected Chinese hamster ovary (CHO) cells with cDNAs for PSGL-1 and
specific glycosyltransferases. CHO cells synthesize only core 1 O-linked glycans (Galβ1 …
human leukocytes. PSGL-1 requires sialylated, fucosylated O-linked glycans and tyrosine
sulfate to bind P-selectin. Less is known about the determinants that PSGL-1 requires to
bind E-selectin. To further define the modifications required for PSGL-1 to bind P-and E-
selectin, we transfected Chinese hamster ovary (CHO) cells with cDNAs for PSGL-1 and
specific glycosyltransferases. CHO cells synthesize only core 1 O-linked glycans (Galβ1 …