[HTML][HTML] Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair

S Frank, B Stallmeyer, H Kämpfer… - The Journal of …, 2000 - Am Soc Clin Investig
S Frank, B Stallmeyer, H Kämpfer, N Kolb, J Pfeilschifter
The Journal of clinical investigation, 2000Am Soc Clin Investig
Wound-healing disorders are a therapeutic problem of extensive clinical importance. Leptin-
deficient ob/ob mice are characterized by a severely delayed wound healing that has been
explained by the mild diabetic phenotype of these animals. Here we demonstrate that
systemically and topically supplemented leptin improved re-epithelialization of wounds in
ob/ob mice. Leptin completely reversed the atrophied morphology of the migrating epithelial
tongue observed at the wound margins of leptin-deficient animals into a well-organized …
Wound-healing disorders are a therapeutic problem of extensive clinical importance. Leptin-deficient ob/ob mice are characterized by a severely delayed wound healing that has been explained by the mild diabetic phenotype of these animals. Here we demonstrate that systemically and topically supplemented leptin improved re-epithelialization of wounds in ob/ob mice. Leptin completely reversed the atrophied morphology of the migrating epithelial tongue observed at the wound margins of leptin-deficient animals into a well-organized hyperproliferative epithelium. Moreover, topically supplemented leptin accelerated normal wound-healing conditions in wild-type mice. As assessed by immunohistochemistry, proliferating keratinocytes located at the wound margins specifically expressed the leptin-receptor subtype ObRb during repair. Additionally, leptin mediated a mitogenic stimulus to the human keratinocyte cell line HaCaT and human primary keratinocytes in vitro. Therefore, leptin might represent an effective novel therapeutic factor to improve impaired wound-healing conditions.
The Journal of Clinical Investigation