Proteasomes regulate erythropoietin receptor and signal transducer and activator of transcription 5 (STAT5) activation: possible involvement of the ubiquitinated Cis …
F Verdier, S Chrétien, O Muller, P Varlet… - Journal of Biological …, 1998 - jbc.org
Cis is an Src homology 2 domain-containing protein, which binds to the erythropoietin
receptor and decreases erythropoietin-stimulated cell proliferation. We show that Cis
associates with the second tyrosine residue of the intracellular domain of the erythropoietin
receptor (Tyr 401). Two forms of Cis with molecular masses of 32 and 37 kDa were detected,
and we demonstrate that the 37-kDa protein resulted from post-translational modifications of
the 32-kDa form. Anti-ubiquitin antibodies recognized the 37-kDa form of Cis and the …
receptor and decreases erythropoietin-stimulated cell proliferation. We show that Cis
associates with the second tyrosine residue of the intracellular domain of the erythropoietin
receptor (Tyr 401). Two forms of Cis with molecular masses of 32 and 37 kDa were detected,
and we demonstrate that the 37-kDa protein resulted from post-translational modifications of
the 32-kDa form. Anti-ubiquitin antibodies recognized the 37-kDa form of Cis and the …
