Formation of reactive nitrogen species during peroxidase-catalyzed oxidation of nitrite: a potential additional mechanism of nitric oxide-dependent toxicity
Involvement of peroxynitrite (ONOO-) in inflammatory diseases has been implicated by
detection of 3-nitrotyrosine, an allegedly characteristic protein oxidation product, in various
inflamed tissues. We show here that nitrite (NO 2-), the primary metabolic end product of
nitric oxide (NO·), can be oxidized by the heme peroxidases horseradish peroxidase,
myeloperoxidase (MPO), and lactoperoxidase (LPO), in the presence of hydrogen peroxide
(H 2 O 2), to most likely form NO· 2, which can also contribute to tyrosine nitration during …
detection of 3-nitrotyrosine, an allegedly characteristic protein oxidation product, in various
inflamed tissues. We show here that nitrite (NO 2-), the primary metabolic end product of
nitric oxide (NO·), can be oxidized by the heme peroxidases horseradish peroxidase,
myeloperoxidase (MPO), and lactoperoxidase (LPO), in the presence of hydrogen peroxide
(H 2 O 2), to most likely form NO· 2, which can also contribute to tyrosine nitration during …
