Effect of pH and bicarbonate on phosphoenolpyruvate carboxykinase and glutaminase mRNA levels in cultured renal epithelial cells
S Kaiser, NP Curthoys - Journal of Biological Chemistry, 1991 - ASBMB
A gluconeogenic strain of renal epithelial cells (LLC-PK1-F+) was used to characterize the
effect of pH and bicarbonate concentration on the levels of phosphoenolpyruvate
carboxykinase (PCK) and glutaminase (GA) mRNAs. The levels of both mRNAs are
markedly dependent upon medium glucose concentration. The level of PCK mRNA is
increased with increasing glucose concentration from 0 to 40 mM, whereas the level of GA
mRNA is maximal between 3 and 5 mM glucose. When LLC-PK1-F+ cells are grown with 5 …
effect of pH and bicarbonate concentration on the levels of phosphoenolpyruvate
carboxykinase (PCK) and glutaminase (GA) mRNAs. The levels of both mRNAs are
markedly dependent upon medium glucose concentration. The level of PCK mRNA is
increased with increasing glucose concentration from 0 to 40 mM, whereas the level of GA
mRNA is maximal between 3 and 5 mM glucose. When LLC-PK1-F+ cells are grown with 5 …