The pro domain of β-secretase does not confer strict zymogen-like properties but does assist proper folding of the protease domain
XP Shi, E Chen, KC Yin, S Na, VM Garsky… - Journal of Biological …, 2001 - jbc.org
β-Secretase (BACE) is a membrane-bound aspartyl protease that cleaves the amyloid
precursor protein to generate the N terminus of the amyloid β peptide. BACE is expressed as
a precursor protein containing Pre, Pro, protease, transmembrane, and cytosolic domains. A
soluble BACE derivative (PreProBACE460) that is truncated between the protease and
transmembrane domains was produced by baculovirus-mediated expression. ProBACE460
was purified from conditioned media of infected insect cells using immobilized concanavalin …
precursor protein to generate the N terminus of the amyloid β peptide. BACE is expressed as
a precursor protein containing Pre, Pro, protease, transmembrane, and cytosolic domains. A
soluble BACE derivative (PreProBACE460) that is truncated between the protease and
transmembrane domains was produced by baculovirus-mediated expression. ProBACE460
was purified from conditioned media of infected insect cells using immobilized concanavalin …