OBJECTIVE: To investigate if cell cycle progression plays a role in modulating the
engraftment potential of mouse hematopoietic stem and progenitor cells (HSPC).
MATERIALS AND METHODS: HSPC were isolated from adult mouse bone marrow, cultured
in vitro under conditions promoting cell cycle arrest, and subsequently were evaluated for
cell cycle status, clonogenic activity, and transplant potential. RESULTS: In the presence of
steel factor (STL) as a survival cytokine, transforming growth factor β (TGF-β) increased the …