Enzymes catalyzing ubiquitination and proteolytic processing of the p105 precursor of nuclear factor κB1
O Coux, AL Goldberg - Journal of Biological Chemistry, 1998 - jbc.org
Nuclear factor κB1 (NF-κB) is a heterodimeric complex that regulates transcription of many
genes involved in immune and inflammatory responses. Its 50-kDa subunit (p50) is
generated by the ubiquitin-proteasome pathway from a 105-kDa precursor (p105). We have
reconstituted this proteolytic process in HeLa cell extracts and purified the responsible
enzymes. Ubiquitination of p105 requires E1, and either of two types of E2s, E2–25K (for
which p105 is the first proven substrate) or a member of the UBCH5 (UBC4) family. It also …
genes involved in immune and inflammatory responses. Its 50-kDa subunit (p50) is
generated by the ubiquitin-proteasome pathway from a 105-kDa precursor (p105). We have
reconstituted this proteolytic process in HeLa cell extracts and purified the responsible
enzymes. Ubiquitination of p105 requires E1, and either of two types of E2s, E2–25K (for
which p105 is the first proven substrate) or a member of the UBCH5 (UBC4) family. It also …
