Preferential utilization of endogenous arachidonate by cyclo-oxygenase in incubations of human platelets
L Sautebin, D Caruso, G Galli, R Paoletti - FEBS letters, 1983 - Elsevier
L Sautebin, D Caruso, G Galli, R Paoletti
FEBS letters, 1983•ElsevierAbstract Thromboxane B 2 (TXB 2) and 12-hydroxy-5, 8, 10, 14-eicosatetraenoic acid (12-
HETE) formed from the endogenous and exogenous arachidonate during human platelet
incubation, was evaluated by selected ion monitoring (SIM). TXB 2 formed from endogenous
substrate accounted for about one third of the total, whereas the great part of 12-HETE
derived from exogenous arachidonate. These data indicate that under the tested conditions
the pool of arachidonate that acts as substrate for cyclo-oxygenase is different from the pool …
HETE) formed from the endogenous and exogenous arachidonate during human platelet
incubation, was evaluated by selected ion monitoring (SIM). TXB 2 formed from endogenous
substrate accounted for about one third of the total, whereas the great part of 12-HETE
derived from exogenous arachidonate. These data indicate that under the tested conditions
the pool of arachidonate that acts as substrate for cyclo-oxygenase is different from the pool …
Abstract
Thromboxane B2 (TXB2) and 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) formed from the endogenous and exogenous arachidonate during human platelet incubation, was evaluated by selected ion monitoring (SIM). TXB2 formed from endogenous substrate accounted for about one third of the total, whereas the great part of 12-HETE derived from exogenous arachidonate. These data indicate that under the tested conditions the pool of arachidonate that acts as substrate for cyclo-oxygenase is different from the pool that acts as substrate for lipoxygenase and that the arachidonate released from phospholipids is preferentially utilized by cyclo-oxygenase.
Elsevier
