The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype.
RM Martin, A Silva, AM Lew - 1997 - cabidigitallibrary.org
RM Martin, A Silva, AM Lew
1997•cabidigitallibrary.orgAbstract Genomic DNA from NOD/Lt mice was amplified by the polymerase chain reaction
(PCR) using primers designed from the 5′ and 3′ flanking sequences conserved in the
Igh-1 genes of C57BL/6, BALB/c and MAI mice. Sequencing of the PCR products showed
that the NOD Igh-1 locus had a similarity of 99.4% to C57BL/6 (IgG2c), 97.2% to MAI IgG2c,
91.8% to MAI IgG2a and 91.5% to BALB/c (IgG2a) sequences. The deduced amino acid
sequence of the NOD gene showed 99.7 and 83.8% similarities to C57BL/6 and MAI …
(PCR) using primers designed from the 5′ and 3′ flanking sequences conserved in the
Igh-1 genes of C57BL/6, BALB/c and MAI mice. Sequencing of the PCR products showed
that the NOD Igh-1 locus had a similarity of 99.4% to C57BL/6 (IgG2c), 97.2% to MAI IgG2c,
91.8% to MAI IgG2a and 91.5% to BALB/c (IgG2a) sequences. The deduced amino acid
sequence of the NOD gene showed 99.7 and 83.8% similarities to C57BL/6 and MAI …
Abstract
Genomic DNA from NOD/Lt mice was amplified by the polymerase chain reaction (PCR) using primers designed from the 5′ and 3′ flanking sequences conserved in the Igh-1 genes of C57BL/6, BALB/c and MAI mice. Sequencing of the PCR products showed that the NOD Igh-1 locus had a similarity of 99.4% to C57BL/6 (IgG2c), 97.2% to MAI IgG2c, 91.8% to MAI IgG2a and 91.5% to BALB/c (IgG2a) sequences. The deduced amino acid sequence of the NOD gene showed 99.7 and 83.8% similarities to C57BL/6 and MAI sequences respectively. The nucleotide sequence of the NOD Igh-1 locus has been submitted to EMBL/GenBank, under accession number Y10606.
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