Isolation of a cell line for rapid and sensitive histochemical assay for the detection of herpes simplex virus
EC Stabell, PD Olivo - Journal of virological methods, 1992 - Elsevier
A cell line which can be used in a simple, sensitive, and rapid histochemical assay was
isolated for detection of herpes simplex virus (HSV). The cell line was derived by selection of
G418 resistant colonies following co-transfection of baby hamster kidney cells with a
plasmid which contains a G418 antibiotic resistance marker and a plasmid which contains
the Escherichia coli LacZ gene placed behind an inducible HSV promoter. The promoter is
from HSV-1UL39 which encodes ICP6, the large subunit of ribonucleotide reductase (RR1) …
isolated for detection of herpes simplex virus (HSV). The cell line was derived by selection of
G418 resistant colonies following co-transfection of baby hamster kidney cells with a
plasmid which contains a G418 antibiotic resistance marker and a plasmid which contains
the Escherichia coli LacZ gene placed behind an inducible HSV promoter. The promoter is
from HSV-1UL39 which encodes ICP6, the large subunit of ribonucleotide reductase (RR1) …