Direct evidence for cross-activation of cGMP-dependent protein kinase by cAMP in pig coronary arteries.
H Jiang, JL Colbran, SH Francis, JD Corbin - Journal of Biological …, 1992 - ASBMB
Elevation of either cAMP or cGMP causes smooth muscle relaxation. Whether these effects
are mediated through cAMP-dependent protein kinase (cAK), cGMP-dependent protein
kinase (cGK), or both is unknown. Pig coronary arteries were treated with sodium
nitroprusside (SNP) or atrial natriuretic factor (ANF), relaxants which elevate cGMP, and with
isoproterenol or forskolin, relaxants which elevate cAMP. Incubation of the arteries with 10
microM SNP produced a 3.3-fold increase in cGMP without altering cAMP; the cGK activity …
are mediated through cAMP-dependent protein kinase (cAK), cGMP-dependent protein
kinase (cGK), or both is unknown. Pig coronary arteries were treated with sodium
nitroprusside (SNP) or atrial natriuretic factor (ANF), relaxants which elevate cGMP, and with
isoproterenol or forskolin, relaxants which elevate cAMP. Incubation of the arteries with 10
microM SNP produced a 3.3-fold increase in cGMP without altering cAMP; the cGK activity …
