Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance.

KM Smith, DC Olson, R Hirose… - International …, 1997 - academic.oup.com
KM Smith, DC Olson, R Hirose, D Hanahan
International immunology, 1997academic.oup.com
We report the initial characterization of rare cells within the thymus that express'
peripheral'self-antigens and are capable of inducing partial tolerance to a model protein.
Mice from two transgenic families that express SV40 T antigen (Tag) in pancreatic islet beta
cells under control of a rat insulin promoter (RIP) develop T cell tolerance toward this neo-
self antigen. These mice express low levels of Tag mRNA in the thymus. Transplantation of
thymus from tolerant RIP-Tag mice into athymic hosts is sufficient to confer tolerance by …
Abstract
We report the initial characterization of rare cells within the thymus that express 'peripheral' self-antigens and are capable of inducing partial tolerance to a model protein. Mice from two transgenic families that express SV40 T antigen (Tag) in pancreatic islet beta cells under control of a rat insulin promoter (RIP) develop T cell tolerance toward this neo-self antigen. These mice express low levels of Tag mRNA in the thymus. Transplantation of thymus from tolerant RIP-Tag mice into athymic hosts is sufficient to confer tolerance by CD4+ Th cells and elicits variable tolerance by CD8+ cytotoxic T cells. Thymic medulla is shown to contain rare cells that express the endogenous insulin and somatostatin genes, and in the transgenic animals, Tag. These cells are referred to as 'peripheral antigen-expressing' (PAE) cells. Thymic cell fractionation reveals the PAE cells expressing insulin and Tag to be present in a fraction enriched for non-lymphoid, MHC class II+ cells. Notably, absence of thymic expression of the RIP-Tag gene in another transgenic family correlates with failure to establish self-tolerance and susceptibility to autoimmunity. Thus, expression of tissue-restricted genes such as insulin in PAE cells of thymic medulla may serve to limit development of potentially autoimmune T cells.
Oxford University Press