Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood

A Bender, M Sapp, G Schuler, RM Steinman… - Journal of …, 1996 - Elsevier
We have investigated an improved method for generating sizable numbers of mature
dendritic cells from nonproliferating progenitors in human blood. The procedure uses 1%
human plasma in the place of 10% fetal calf serum and involves two steps. The first step or
'priming'phase is a 6–7 day culture of T cell depleted mononuclear cells in medium
supplemented with GM-CSF and IL-4. The second step or 'differentiation'phase requires the
exposure to macrophage conditioned medium. This medium cannot be replaced by several …