Detection and quantification of blood-derived CD8+ T lymphocytes secreting tumor necrosis factor α in response to HLA-A2. 1-binding melanoma and viral peptide …
W Herr, J Schneider, AW Lohse… - Journal of …, 1996 - Elsevier
We applied an enzyme-linked immunospot (ELISPOT) assay for the detection and
quantification of blood-derived CD8+ T cells recognizing peptide antigens presented by HLA-
A2. 1. CD8+ T lymphocytes were isolated from peripheral blood and were stimulated for 40 h
with peptide-loaded A2. 1-positive 0.174× CEM. T2 cells. Tumor necrosis factor α (TNF-α)
secreted by single T cells in response to antigen contact was trapped on nitrocellulose
membranes precoated with anti-TNF-α antibodies and was then immunochemically …
quantification of blood-derived CD8+ T cells recognizing peptide antigens presented by HLA-
A2. 1. CD8+ T lymphocytes were isolated from peripheral blood and were stimulated for 40 h
with peptide-loaded A2. 1-positive 0.174× CEM. T2 cells. Tumor necrosis factor α (TNF-α)
secreted by single T cells in response to antigen contact was trapped on nitrocellulose
membranes precoated with anti-TNF-α antibodies and was then immunochemically …
