Nucleosome‐restricted antibodies are detected before anti‐dsDNA and/or antihistone antibodies in serum of MRL‐Mp lpr/lpr and+/+ mice, and are present in kidney …
Z Amoura, H Chabre, S Koutouzov… - … : Official Journal of …, 1994 - Wiley Online Library
Arthritis & Rheumatism: Official Journal of the American College …, 1994•Wiley Online Library
Objective. To compare the humoral response to nucleosomes with the response to their
individual components (double‐stranded DNA [dsDNA] and histones) and to assess the
involvement of antinucleosome antibodies in immune deposits in the kidney of MRL mice.
Methods. We used enzyme‐linked immunosorbent assays of sera and kidney eluates for
antibody activity against purified nucleosomes, dsDNA, and histones. Results.
Antinucleosome antibodies emerged before anti‐dsDNA and antihistone antibodies. A …
individual components (double‐stranded DNA [dsDNA] and histones) and to assess the
involvement of antinucleosome antibodies in immune deposits in the kidney of MRL mice.
Methods. We used enzyme‐linked immunosorbent assays of sera and kidney eluates for
antibody activity against purified nucleosomes, dsDNA, and histones. Results.
Antinucleosome antibodies emerged before anti‐dsDNA and antihistone antibodies. A …
Abstract
Objective. To compare the humoral response to nucleosomes with the response to their individual components (double‐stranded DNA [dsDNA] and histones) and to assess the involvement of antinucleosome antibodies in immune deposits in the kidney of MRL mice.
Methods. We used enzyme‐linked immunosorbent assays of sera and kidney eluates for antibody activity against purified nucleosomes, dsDNA, and histones.
Results. Antinucleosome antibodies emerged before anti‐dsDNA and antihistone antibodies. A fraction of antinucleosome antibodies reacted exclusively with nucleosomes and not with their components, dsDNA and histones. These nucleosome‐restricted antibodies were detected in the proteinuric MRL mouse kidney eluate.
Conclusion. Our findings support the notion that nucleosomes play a major role in the emergence of antinuclear autoantibodies and that antinucleosome antibodies might be involved in the nephritogenic process in murine lupus.
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