Human autoantibodies against a desmosomal core protein in pemphigus foliaceus.
L Koulu, A Kusumi, MS Steinberg… - Journal of Experimental …, 1984 - rupress.org
L Koulu, A Kusumi, MS Steinberg, V Klaus-Kovtun, JR Stanley
Journal of Experimental Medicine, 1984•rupress.orgMaterials and Methods Human Epidermal and Desmosomal Extracts. Normal human
epidermis, obtained by excising the roofs of suction blisters induced on the volar forearm of
volunteers, was extracted with sample buffer (2% SDS, 0.0625 M Tris-HCI, pH 6.8)(14).
Desmosomes were isolated from bovine muzzle using nonionic detergent in citrate buffer
and differential centrifugation, as previously described (7). The desmosomal pellet was
extracted with sample buffer in preparation for SDS-PAGE.
epidermis, obtained by excising the roofs of suction blisters induced on the volar forearm of
volunteers, was extracted with sample buffer (2% SDS, 0.0625 M Tris-HCI, pH 6.8)(14).
Desmosomes were isolated from bovine muzzle using nonionic detergent in citrate buffer
and differential centrifugation, as previously described (7). The desmosomal pellet was
extracted with sample buffer in preparation for SDS-PAGE.
Materials and Methods
Human Epidermal and Desmosomal Extracts. Normal human epidermis, obtained by excising the roofs of suction blisters induced on the volar forearm of volunteers, was extracted with sample buffer (2% SDS, 0.0625 M Tris-HCI, pH 6.8)(14). Desmosomes were isolated from bovine muzzle using nonionic detergent in citrate buffer and differential centrifugation, as previously described (7). The desmosomal pellet was extracted with sample buffer in preparation for SDS-PAGE.
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