STING activation reprograms the microenvironment to sensitize NF1-related malignant peripheral nerve sheath tumors for immunotherapy
Bandarigoda N. Somatilaka, … , Renee M. McKay, Lu Q. Le
Bandarigoda N. Somatilaka, … , Renee M. McKay, Lu Q. Le
Published March 19, 2024
Citation Information: J Clin Invest. 2024;134(10):e176748. https://doi.org/10.1172/JCI176748.
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Research Article Oncology Therapeutics

STING activation reprograms the microenvironment to sensitize NF1-related malignant peripheral nerve sheath tumors for immunotherapy

Abstract

Neurofibromatosis type 1 (NF1) is caused by mutations in the NF1 gene that encodes neurofibromin, a RAS GTPase–activating protein. Inactivating NF1 mutations cause hyperactivation of RAS-mediated signaling, resulting in the development of multiple neoplasms, including malignant peripheral nerve sheath tumors (MPNSTs). MPNSTs are an aggressive tumor and the main cause of mortality in patients with NF1. MPNSTs are difficult to resect and refractory to chemo- and radiotherapy, and no molecular therapies currently exist. Immune checkpoint blockade (ICB) is an approach to treat inoperable, undruggable cancers like MPNST, but successful outcomes require an immune cell–rich tumor microenvironment. While MPNSTs are noninflamed “cold” tumors, here, we converted MPNSTs into T cell–inflamed “hot” tumors by activating stimulator of IFN genes (STING) signaling. Mouse genetic and human xenograft MPNST models treated with a STING agonist plus ICB exhibited growth delay via increased apoptotic cell death. This strategy offers a potential treatment regimen for MPNSTs.

Authors

Bandarigoda N. Somatilaka, Laasya Madana, Ali Sadek, Zhiguo Chen, Sanjay Chandrasekaran, Renee M. McKay, Lu Q. Le

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Figure 6

Combination treatment of xenograft human MPNST with a STING agonist plus ICB accelerates complete tumor regression.

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Combination treatment of xenograft human MPNST with a STING agonist plu...
(A) Schema showing the design of the mouse xenograft MPNST model and the treatment regimen of ADU-S100 with ICB. (B) Change in xenograft MPNST volumes following the indicated treatments. Control, ADU-S100, and ADU-S100 plus anti–PD-1 (n = 15 each). (C) Paraffin sections from xenograft MPNSTs treated as indicated were stained for T cell markers. (D) Paraffin sections from xenograft MPNSTs treated as indicated were stained for cleaved caspase 3 and cleaved PARP. Control, n = 3–4; ADU-S100, n = 3–4; ADU-S100 plus anti–PD-1, n = 3–4. Data are presented as the mean ± SEM. *P < 0.05 and **P < 0.01, by Tukey’s multiple-comparison test. Scale bars: 50 μm. Original magnification, ×80 (enlarged insets in C and D).