Human neutrophils triggered with phorbol myristate acetate or opsonized zymosan particles released a metalloproteinase (MP) capable of cleaving and inactivating alpha-1-proteinase inhibitor (alpha-1-PI). Sequence analysis of the amino acids in proteolyzed, native alpha-1-PI revealed a unique single cleavage site between Phe-352 and Leu-353. An analysis of the process regulating the enzyme's activity revealed that the neutrophil MP was released from cells in a latent form whose activation was tightly linked to the generation of hypochlorous acid. These results indicate that human neutrophils use chlorinated oxidants to activate a latent MP that is capable of proteolytically inactivating alpha-1-PI by cleaving the antiproteinase at a unique point in its inhibitory site region.
P E Desrochers, S J Weiss
Title and authors | Publication | Year |
---|---|---|
Protease allergens as initiators-regulators of allergic inflammation.
Soh WT, Zhang J, Hollenberg MD, Vliagoftis H, Rothenberg ME, Sokol CL, Robinson C, Jacquet A |
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Multifunctional Role of Chymase in Acute and Chronic Tissue Injury and Remodeling
LJ DellItalia, JF Collawn, CM Ferrario |
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Therapeutic targeting of cathepsin C: from pathophysiology to treatment
B Korkmaz, GH Caughey, I Chapple, F Gauthier, J Hirschfeld, DE Jenne, R Kettritz, G Lalmanach, AS Lamort, C Lauritzen, M Łȩgowska, A Lesner, S Marchand-Adam, SJ McKaig, C Moss, J Pedersen, H Roberts, A Schreiber, S Seren, NS Thakker |
Pharmacology & Therapeutics | 2018 |
Chemically treated plasma Aβ is a potential blood-based biomarker for screening cerebral amyloid deposition
JC Park, SH Han, HJ Cho, MS Byun, D Yi, YM Choe, S Kang, ES Jung, SJ Won, EH Kim, YK Kim, DY Lee, I Mook-Jung |
Alzheimer's research & therapy | 2017 |
Handbook of Proteolytic Enzymes
FS Markland, S Swenson |
Handbook of Proteolytic Enzymes | 2013 |
Therapeutic effect of lecithinized superoxide dismutase on pulmonary emphysema
KI Tanaka, Y Tanaka, Y Miyazaki, T Namba, K Sato, K Aoshiba, A Azuma, T Mizushima |
The Journal of pharmacology and experimental therapeutics | 2011 |
Annual Reports in Medicinal Chemistry
JJ Liu, TW Lee |
Annual Reports in Medicinal Chemistry Volume 46 | 2011 |
Roles for proteinases in the pathogenesis of chronic obstructive pulmonary disease
CA Owen |
International journal of chronic obstructive pulmonary disease | 2008 |
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J Münch, L Ständker, K Adermann, A Schulz, M Schindler, R Chinnadurai, S Pöhlmann, C Chaipan, T Biet, T Peters, B Meyer, D Wilhelm, H Lu, W Jing, S Jiang, WG Forssmann, F Kirchhoff |
Cell | 2007 |
Myeloperoxidase and its contributory role in inflammatory vascular disease
D Lau, S Baldus |
Pharmacology & Therapeutics | 2006 |
Superoxide dismutase expression attenuates cigarette smoke- or elastase-generated emphysema in mice
RF Foronjy, O Mirochnitchenko, O Propokenko, V Lemaitre, Y Jia, M Inouye, Y Okada, JM D'Armiento |
American journal of respiratory and critical care medicine | 2005 |
Inhibition of neutrophil elastase by alpha1-protease inhibitor at the surface of human polymorphonuclear neutrophils
B Korkmaz, S Attucci, ML Jourdan, L Juliano, F Gauthier |
Journal of immunology (Baltimore, Md. : 1950) | 2005 |
Proteolytic-antiproteolytic balance and its regulation in carcinogenesis
Elzbieta Skrzydlewska, Mariola Sulkowska, Mariusz Koda, Stanislaw Sulkowski |
World journal of gastroenterology : WJG | 2005 |
Direct Tandem Mass Spectrometry Reveals Limitations in Protein Profiling Experiments for Plasma Biomarker Discovery
JM Koomen, D Li, L Xiao, TC Liu, KR Coombes, J Abbruzzese, R Kobayashi |
Journal of Proteome Research | 2005 |
Elastase Release by Transmigrating Neutrophils Deactivates Endothelial-bound SDF-1α and Attenuates Subsequent T Lymphocyte Transendothelial Migration
RM Rao, TV Betz, DJ Lamont, MB Kim, SK Shaw, RM Froio, F Baleux, F Arenzana-Seisdedos, R Alon, FW Luscinskas |
Journal of Experimental Medicine | 2004 |
Use of Modulators of Airways Inflammation in Patients with CF
CL Ren |
Clinical Reviews in Allergy & Immunology | 2002 |
Endothelial dysfunction is induced by proinflammatory oxidant hypochlorous acid
C Zhang, R Patel, JP Eiserich, F Zhou, S Kelpke, W Ma, DA Parks, V Darley-Usmar, CR White |
American journal of physiology. Heart and circulatory physiology | 2001 |
Serine proteases increase oxidative stress in lung cells
K Aoshiba, K Yasuda, S Yasui, J Tamaoki, A Nagai |
American journal of physiology. Lung cellular and molecular physiology | 2001 |
DNA from Bronchial Secretions Modulates Elastase Inhibition by α 1 -Proteinase Inhibitor and Oxidized Secretory Leukoprotease Inhibitor
QL Ying, SR Simon |
American journal of respiratory cell and molecular biology | 2000 |
Handbook of Oxidants and Antioxidants in Exercise
RR Jenkins, J Beard |
Handbook of Oxidants and Antioxidants in Exercise | 2000 |
Inhibition of human serine proteases by SPAAT, the C-terminal 44-residue peptide from α1-antitrypsin
MA Niemann, JE Baggott, EJ Miller |
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology | 1997 |
Neutral proteinases and their inhibitors in the loosening of total hip prostheses
M Takagi |
Acta Orthopaedica | 1996 |
ARDS Acute Respiratory Distress in Adults
TW Evans, C Haslett |
1996 | |
Fc gamma receptor IIIb enhances Fc gamma receptor IIa function in an oxidant-dependent and allele-sensitive manner
JE Salmon, SS Millard, NL Brogle, RP Kimberly |
Journal of Clinical Investigation | 1995 |
Monitoring of immunotherapy with cytokines or monoclonal antibodies
CE Hack |
Cytotechnology | 1995 |
Oxidation of methionyl residues in proteins: Tools, targets, and reversal
W Vogt |
Free radical biology & medicine | 1995 |
α1-Antitrypsin Is Degraded and Non-Functional in Chronic Wounds But Intact and Functional in Acute Wounds: The Inhibitor Protects Fibronectin from Degradation by Chronic Wound Fluid Enzymes
CN Rao, DA Ladin, YY Liu, K Chilukuri, ZZ Hou, DT Woodley |
Journal of Investigative Dermatology | 1995 |
Elastase activity, uninhibited by ?1-antitrypsin, in the periprosthetic connective matrix around loose total hip prostheses
M Takagi, YT Konttinen, S Santavirta, P Kangaspunta, T Sorsa, M Yamakawa, A Suda |
Journal of Orthopaedic Research | 1995 |
Immunopharmacology of Free Radical Species
C Rice-Evans |
Immunopharmacology of Free Radical Species | 1995 |
Animal Cell Technology: Developments Towards the 21st Century
EC Beuvery, JB Griffiths, WP Zeijlemaker |
1995 | |
Proteolytic inactivation of alpha-1-antitrypsin by human neutrophils: involvement of multiple and interlinked cell responses to phagocytosable targets
L Ottonello, P Dapino, M Scirocco, F Dallegri, C Sacchetti |
European Journal of Clinical Investigation | 1994 |
The Contribution of Hypoxia-Reperfusion Injury to Inflammatory Synovitis: The Influence of Reactive Oxygen Intermediates on the Transcriptional Control of Inflammation
DR Blake, PG Winyard, R Marok |
Annals of the New York Academy of Sciences | 1994 |
Cloning and Characterization of Human Tissue Inhibitor of Metalloproteinases-3
CG Wilde, PR Hawkins, RT Coleman, WB Levine, AM Delegeane, PM Okamoto, LY Ito, RW Scott, JJ Seilhamer |
DNA and Cell Biology | 1994 |
Proteolysis of human native and oxidised α1-proteinase inhibitor by matrulysin and stromelysin
Z Zhang, PG Winyard, K Chidwick, G Murphy, M Wardell, CR W., DR Blake |
Biochimica et Biophysica Acta (BBA) - General Subjects | 1994 |
Recombinant C1 inhibitor P5/P3 variants display resistance to catalytic inactivation by stimulated neutrophils
E Eldering, CC Huijbregts, JH Nuijens, AJ Verhoeven, CE Hack |
Journal of Clinical Investigation | 1993 |
Proteolytic inactivation of α1-antitrypsin and α1-antichymotrypsin by neutrophils in arthritic joints
JJ Abbink, AM Kamp, JH Nuijens, TJ Swaak, CE Hack |
Arthritis & Rheumatism | 1993 |
Human α 1 -Proteinase Inhibitor Binds to Extracellular Matrix In Vitro
AR Rinehart, S Mallya, SR Simon |
American journal of respiratory cell and molecular biology | 1993 |
Oxidized and mutated inhibitor as substrates of Pseudomonas aeruginosa elastase
M Padrines, JG Bieth |
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology | 1993 |
Opossum serum .alpha.1-proteinase inhibitor: Purification, linear sequence, and resistance to inactivation by rattlesnake venom metalloproteinase
JJ Catanese, LF Kress |
Biochemistry | 1993 |
Shock, Sepsis, and Organ Failure
G Schlag, H Redl, D Traber |
1993 | |
Proteases, Protease Inhibitors and Protease-Derived Peptides
JC Cheronis, JE Repine |
1993 | |
Identification and purification of a novel serine proteinase inhibitor
PB Coughlin, T Tetaz, HH Salem |
The Journal of biological chemistry | 1993 |
Inhibition of neutrophil superoxide production by human plasma α1-antitrypsin
N Bucurenci, DR Blake, K Chidwick, PG Winyard |
FEBS Letters | 1992 |
Specificity of the anticollagenase action of tetracyclines: relevance to their anti-inflammatory potential
K Suomalainen, T Sorsa, LM Golub, N Ramamurthy, HM Lee, VJ Uitto, H Saari, YT Konttinen |
Antimicrobial agents and chemotherapy | 1992 |
Enzyme-linked immunosorbent assay for proteolytically inactivated antithrombin-III: Use of sodium dodecyl sulfate to eliminate signal due to intact antithrombin-III
PC Esmon, E Yee |
Analytical Biochemistry | 1992 |
The serine proteinase inhibitory proteins of the human intervertebral disc: Their isolation, characterization and variation with ageing and degeneration
J Melrose, P Ghosh, TK Taylor, JL Andrews |
Matrix | 1992 |
Biochemistry of Pulmonary Emphysema
C Grassi, J Travis, L Casali, M Luisetti |
1992 | |
Biochemistry of Inflammation
JT Whicher, SW Evans |
1992 | |
Akademische Chirurgie
L Schweiberer, JR Izbicki |
1992 | |
Proteolytic inactivation of alpha 1-proteinase inhibitor and alpha 1-antichymotrypsin by oxidatively activated human neutrophil metalloproteinases
PE Desrochers, K Mookhtiar, HE Wart, KA Hasty, SJ Weiss |
The Journal of biological chemistry | 1992 |
Interstitial collagenase (matrix metalloproteinase-1) expresses serpinase activity
PE Desrochers, JJ Jeffrey, SJ Weiss |
Journal of Clinical Investigation | 1991 |
Proteolytic enzymes as mediators of glomerular injury
WH Baricos, SV Shah |
Kidney International | 1991 |
The cellular basis for liver injury in α1-antitrypsin deficiency
DH Perlmutter |
Hepatology | 1991 |
Predominant Role of Neutrophils in the Inactivation of α2-Macroglobulin in Arthritic Joints
JJ Abbink, AM Kamp, EJ Nieuwenhuys, JH Nuijens, AJ Swaak, CE Hack |
Arthritis & Rheumatism | 1991 |
Oxidative Inactivation of α 1 -Proteinase Inhibitor by Alveolar Macrophages from Healthy Smokers Requires the Presence of Myeloperoxidase
B Wallaert, B Gressier, C Aerts, C Mizon, C Voisin, J Mizon |
American journal of respiratory cell and molecular biology | 1991 |
Tetracyclines Inhibit Connective Tissue Breakdown: New Therapeutic Implications for an Old Family of Drugs
LM Golub, NS Ramamurthy, TF McNamara, RA Greenwald, BR Rifkin |
Critical Reviews in Oral Biology & Medicine | 1991 |
Sequence specificities of human fibroblast and neutrophil collagenases
S Netzel-Arnett, GB Fields, H Birkedal-Hansen, HE Wart, G Fields |
The Journal of biological chemistry | 1991 |
Kinetics and physiologic relevance of the inactivation of alpha 1-proteinase inhibitor, alpha 1-antichymotrypsin, and antithrombin III by matrix metalloproteinases-1 (tissue collagenase), -2 (72-kDa gelatinase/type IV collagenase), and -3 (stromelysin)
AE Mast, JJ Enghild, H Nagase, K Suzuki, SV Pizzo, G Salvesen |
The Journal of biological chemistry | 1991 |
Study of human epithelial cell detachment and damage: Effects of proteases and oxidants
AH Mendis, TJ Venaille, BW Robinson |
Immunology and Cell Biology | 1990 |
Human neutrophil collagenase cleaves alpha 1-antitrypsin
J Michaelis, MC Vissers, CC Winterbourn |
Biochemical Journal | 1990 |
Inactivation of human plasma α1-proteinase inhibitor by human PMN leucocyte collagenase
V Knäuper, H Reinke, H Tschesche |
FEBS Letters | 1990 |
Proteolytic activities of human fibroblast collagenase: hydrolysis of a broad range of substrates at a single active site
GB Fields, SJ Netzel-Arnett, LJ Windsor, JA Engler, H Birkedal-Hansen, HE van Wart |
Biochemistry | 1990 |
Pathophysiology of Severe Ischemic Myocardial Injury
HM Piper |
1990 | |
Regulation of Proteolysis at the Neutrophil-Substrate Interface by Secretory Leukoprotease Inhibitor
WG Rice, SJ Weiss |
Science | 1990 |
Disruption of the subendothelial basement membrane during neutrophil diapedesis in an in vitro construct of a blood vessel wall
AR Huber, SJ Weiss |
Journal of Clinical Investigation | 1989 |
Interactions between Oxygen Free Radicals and ProteinsImplications for Rheumatoid Arthritis. An Overview
HA Kleinveld, AJ Swaak, CE Hack, JF Koster |
Scandinavian Journal of Rheumatology | 1989 |
Granulocyte elastase-mediated proteolysis of alpha1-antitrypsin in cystic fibrosis bronchopulmonary secretions
A Cantin, G Bilodeau, R Bégin |
Pediatric Pulmonology | 1989 |
Inhibition of Neutrophil Elastase by Alpha-1-Proteinase Inhibitor Oxidized by Activated Neutrophils
M Padrines, M Schneider-Pozzer, JG Bieth |
American journal of respiratory and critical care medicine | 1989 |
Acute Phase Proteins in the Acute Phase Response
MB Pepys |
1989 | |
Functional Inactivation and Structural Disruption of Human α2-Macroglobulin by Neutrophils and Eosinophils
VY Reddy, SV Pizzo, SJ Weiss |
The Journal of biological chemistry | 1989 |
Adult Respiratory Distress Syndrome
JE Rinaldo, RM Rogers |
New England Journal of Medicine | 1986 |
Progress in Medicinal Chemistry
JR Brown, SH Imam |
Progress in Medicinal Chemistry | 1985 |
New Comprehensive Biochemistry
S Yamamoto |
New Comprehensive Biochemistry | 1983 |
Cell Biology of Extracellular Matrix
ED Hay |
1981 |