Proteomic profile associated with loss of spontaneous human immunodeficiency virus type 1 elite control
E Rodríguez-Gallego, L Tarancón-Diez… - The Journal of …, 2019 - academic.oup.com
E Rodríguez-Gallego, L Tarancón-Diez, F García, J Del Romero, JM Benito, V Alba…
The Journal of infectious diseases, 2019•academic.oup.comAbstract Background Elite controllers (ECs) spontaneously control plasma human
immunodeficiency virus type 1 (HIV-1) RNA without antiretroviral therapy. However, 25%
lose virological control over time. The aim of this work was to study the proteomic profile that
preceded this loss of virological control to identify potential biomarkers. Methods Plasma
samples from ECs who spontaneously lost virological control (transient controllers [TCs]), at
2 years and 1 year before the loss of control, were compared with a control group of ECs …
immunodeficiency virus type 1 (HIV-1) RNA without antiretroviral therapy. However, 25%
lose virological control over time. The aim of this work was to study the proteomic profile that
preceded this loss of virological control to identify potential biomarkers. Methods Plasma
samples from ECs who spontaneously lost virological control (transient controllers [TCs]), at
2 years and 1 year before the loss of control, were compared with a control group of ECs …
Background
Elite controllers (ECs) spontaneously control plasma human immunodeficiency virus type 1 (HIV-1) RNA without antiretroviral therapy. However, 25% lose virological control over time. The aim of this work was to study the proteomic profile that preceded this loss of virological control to identify potential biomarkers.
Methods
Plasma samples from ECs who spontaneously lost virological control (transient controllers [TCs]), at 2 years and 1 year before the loss of control, were compared with a control group of ECs who persistently maintained virological control during the same follow-up period (persistent controllers [PCs]). Comparative plasma shotgun proteomics was performed with tandem mass tag (TMT) isobaric tag labeling and nanoflow liquid chromatography coupled to Orbitrap mass spectrometry.
Results
Eighteen proteins exhibited differences comparing PC and preloss TC timepoints. These proteins were involved in proinflammatory mechanisms, and some of them play a role in HIV-1 replication and pathogenesis and interact with structural viral proteins. Coagulation factor XI, α-1-antichymotrypsin, ficolin-2, 14-3-3 protein, and galectin-3-binding protein were considered potential biomarkers.
Conclusions
The proteomic signature associated with the spontaneous loss of virological control was characterized by higher levels of inflammation, transendothelial migration, and coagulation. Galectin-3 binding protein could be considered as potential biomarker for the prediction of virological progression and as therapeutic target in ECs.
Oxford University Press