Deep brain optogenetics without intracranial surgery
Nature biotechnology, 2021•nature.com
Achieving temporally precise, noninvasive control over specific neural cell types in the deep
brain would advance the study of nervous system function. Here we use the potent
channelrhodopsin ChRmine to achieve transcranial photoactivation of defined neural
circuits, including midbrain and brainstem structures, at unprecedented depths of up to 7 mm
with millisecond precision. Using systemic viral delivery of ChRmine, we demonstrate
behavioral modulation without surgery, enabling implant-free deep brain optogenetics.
brain would advance the study of nervous system function. Here we use the potent
channelrhodopsin ChRmine to achieve transcranial photoactivation of defined neural
circuits, including midbrain and brainstem structures, at unprecedented depths of up to 7 mm
with millisecond precision. Using systemic viral delivery of ChRmine, we demonstrate
behavioral modulation without surgery, enabling implant-free deep brain optogenetics.
Abstract
Achieving temporally precise, noninvasive control over specific neural cell types in the deep brain would advance the study of nervous system function. Here we use the potent channelrhodopsin ChRmine to achieve transcranial photoactivation of defined neural circuits, including midbrain and brainstem structures, at unprecedented depths of up to 7 mm with millisecond precision. Using systemic viral delivery of ChRmine, we demonstrate behavioral modulation without surgery, enabling implant-free deep brain optogenetics.
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