[HTML][HTML] Characterization of the ligand binding functionality of the extracellular domain of activin receptor type IIb

D Sako, AV Grinberg, J Liu, MV Davies… - Journal of Biological …, 2010 - ASBMB
The single transmembrane domain serine/threonine kinase activin receptor type IIB
(ActRIIB) has been proposed to bind key regulators of skeletal muscle mass development,
including the ligands GDF-8 (myostatin) and GDF-11 (BMP-11). Here we provide a detailed
kinetic characterization of ActRIIB binding to several low and high affinity ligands using a
soluble activin receptor type IIB-Fc chimera (ActRIIB. Fc). We show that both GDF-8 and GDF-
11 bind the extracellular domain of ActRIIB with affinities comparable with those of activin A …