[HTML][HTML] Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro
JN Rauch, JE Gestwicki - Journal of Biological Chemistry, 2014 - ASBMB
Proteins with Bcl2-associated anthanogene (BAG) domains act as nucleotide exchange
factors (NEFs) for the molecular chaperone heat shock protein 70 (Hsp70). There are six
BAG family NEFs in humans, and each is thought to link Hsp70 to a distinct cellular pathway.
However, little is known about how the NEFs compete for binding to Hsp70 or how they
might differentially shape its biochemical activities. Toward these questions, we measured
the binding of human Hsp72 (HSPA1A) to BAG1, BAG2, BAG3, and the unrelated NEF …
factors (NEFs) for the molecular chaperone heat shock protein 70 (Hsp70). There are six
BAG family NEFs in humans, and each is thought to link Hsp70 to a distinct cellular pathway.
However, little is known about how the NEFs compete for binding to Hsp70 or how they
might differentially shape its biochemical activities. Toward these questions, we measured
the binding of human Hsp72 (HSPA1A) to BAG1, BAG2, BAG3, and the unrelated NEF …